A) Stock solutions for BG-11:
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Stock 1:
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Na2MG EDTA
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0.1g/liter |
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Ferric ammonium citrate
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0.6g/liter |
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Citric acid . 1H2O
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0.6g/liter |
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CaCl2 . 2H2O
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3.6g/liter |
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Filter sterilize into a sterile bottle or autoclave
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Stock 2:
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MgSO4 . 7H2O
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7.5g/liter |
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Filter sterilize into a sterile bottle or autoclave
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Stock 3:
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K2HPO4 . 3H2O
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4.0g/liter |
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or K2HPO4
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3.05g/liter |
| Filter sterilize into a sterile bottle or autoclave |
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Stock 5 (Microelements):
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H3BO3
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2.86g/liter |
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MnCl2 . 4H2O
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1.81g/liter |
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ZnSO4 . 7H2O
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0.222g/liter |
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CuSO4 . 5H2O
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0.079g/liter |
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COCl2 . 6H2O
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0.050g/liter |
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NaMoO4 . 2H2O
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0.391g/liter |
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or MoO4 (85%)
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0.018g/liter |
B) For basic BG11 medium combine the following stock solutions:
| Stock Solution |
Per Liter of medium |
| Stock 1 |
10 ml
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| Stock 2 |
10 ml
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| Stock 3 |
10 ml
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| Na2CO3 |
0.02g
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| Stock 5 |
1.0 ml
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| NaNO3 |
1.5g
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| Combine stocks and adjust pH to 7.5 (use 1.0N HCl). Aliquot into flasks (50 ml/125 ml flask) with cotton stoppers on top and autoclave. After autoclaving and cooling the pH is about 7.1. For solid media add 1% noble agar. For BG-11o don't add NaNO3. |
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